Sains Malaysiana 52(12)(2023): 3421-3435
http://doi.org/10.17576/jsm-2023-5212-06
Physicochemical
Properties of Raw Cleaned Edible Bird’s Nest after Different Primary Processing
Including a New Cleaning Method
(Sifat Fizikokimia Sarang Burung Boleh Dimakan Mentah yang Dibersihkan Selepas Pemprosesan Utama Berbeza Termasuk Kaedah Pembersihan Baharu)
BEE-HUI YEO1,
YAYA RUKAYADI1, SHEW-FUNG WONG2, CHIN-PING TAN3 & OI-MING LAI1,4,*
1Natural Medicines
and Products Research Laboratory (NaturMeds),
Institute of Bioscience, Universiti Putra Malaysia,
43400 UPM Serdang, Selangor, Malaysia
2School
of Medicine, International Medical University, 57000 Kuala Lumpur, Malaysia
3Department
of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
4Department
of Bioprocess Technology, Faculty of Biotechnology & Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
Diserahkan: 28 Mei 2023/Diterima: 17
November 2023
Abstract
Primary
processing is the cleaning process of raw uncleaned (RUC) edible bird's nest
(EBN) into raw clean (RC) bird's nest. In this study, three primary
processing methods were used, including two methods commonly used in industry
(semi-dry and wet methods) and a newly proposed method (semi-wet method). EBNs
before and after primary processing were characterised by structural and
chemical analysis. The RC EBN samples after initial processing were divided
into two categories, with moulding (cup-shaped) and un-shaped. Scanning
Electron Microscopy-Energy Dispersive X-ray Microanalysis (SEM-EDX) was used to
study the morphology and element composition of EBN samples. The EBN sample
were also tested on their colour, moisture content, water activity, nitrite
content, nitrate content, total sialic acid content, total glycoprotein
content, and total polysaccharide content. The semi-wet method yielded > 75%
cup- shaped RC EBN for six different batches of RUC EBN, outperforming wet
cleaning method (68.76 - 82.92%). Micrographs showed structural similarity
between RC EBN samples even with different cleaning methods; but showed
structural difference between RUC and RC EBN samples. Elements Na and Cl were
significantly (P < 0.05) higher in RUC EBN. After cleaning, RC EBNs showed
nitrite reduction at 34.78 - 60.72%; and nitrate reduction at 33.62 - 72.35%,
respectively. No reduction of antioxidant activity, total sialic acid content,
total glycoprotein content and total polysaccharide were observed for EBNs
after primary processing. The nitrate and glycoprotein contents in EBNs after
moulding were lower than those before moulding.
Keywords:
Edible bird’s nest; glycoprotein; primary processing; sialic acid
Abstrak
Pemprosesan primer ialah proses pembersihan sarang burung walit (EBN) mentah (RUC) kepada sarang burung walit mentah bersih (RC). Dalam kajian ini, tiga kaedah pemprosesan utama telah digunakan, termasuk dua kaedah yang biasa digunakan dalam industri (kaedah separa kering dan basah) dan kaedah yang baharu dicadangkan (kaedah separa basah).
EBN sebelum dan selepas pemprosesan utama dicirikan oleh analisis struktur dan kimia. Sampel RC EBN selepas pemprosesan primer dibahagikan kepada dua kategori dengan pengacuan (berbentuk cawan) dan tidak berbentuk. Mikroskop elektron pengimbas - X-ray Penyerakan Tenaga Microanalisis (SEM-EDX) digunakan untuk mengkaji morfologi dan komposisi unsur sampel EBN. Sampel EBN juga diuji warna, kandungan lembapan, aktiviti air, kandungan nitrit, kandungan nitrat, jumlah kandungan asid sialik, jumlah kandungan glikoprotein dan jumlah kandungan polisakaridanya. Kaedah separa basah menghasilkan > 75% RC EBN berbentuk cawan untuk enam kelompok RUC EBN yang berbeza, mengatasi kaedah pembersihan basah (68.76 -
82.92%). Mikrograf menunjukkan persamaan struktur antara sampel RC EBN walaupun dengan kaedah pembersihan yang berbeza; tetapi menunjukkan perbezaan struktur antara sampel RUC dan RC EBN. Unsur Na dan Cl adalah ketara (P <0.05) tertinggi dalam RUC EBN. Selepas pembersihan, RC EBN telah menunjukkan pengurangan nitrit pada 34.78 - 60.72%; dan pengurangan nitrat pada 33.62 - 72.35%. Tiada pengurangan aktiviti antioksidan, jumlah kandungan asid sialik, jumlah kandungan glikoprotein dan jumlah polisakarida diperhatikan di EBN selepas pemprosesan primer. Kandungan nitrat dan glikoprotein dalam EBN selepas pengacuan adalah lebih rendah daripada sebelum pengacuan.
Kata kunci: Asid sialik; glikoprotein; pemprosesan primer; sarang burung walit
rujukan
Babji, A.S., Nurfatin, M.H., Etty Syarmila, I.K. & Masitah, M. 2015. Secrets of edible bird nest. Agriculture
Science 1: 32-37.
Bai, W., Liu, X., Fan, Q., Lian, J. & Guo, B. 2023.
Study of the antiaging effects of bird’s nest peptide based on biochemical,
cellular, and animal models. Journal of Functional Foods 103: 105479.
Chee, B.M. 2018. Three
dimensional printing of biotic material originated from swiftlet edible bird
nest. China CN108260839A, filed January 3, 2018, and issued July 10, 2018.
https://patents.google.com/patent/CN108260839A/en.
Chantakun, K., Kishimura, H., Kumagai, Y. & Benjakul, S.
2022. Physicochemical properties of house and cave edible bird’s nest from
Southern Thailand. ScienceAsia 48: 136-143.
Dai, Y., Cao, J., Wang, Y.,
Chen, Y. & Jiang, L. 2021. A comprehensive review of edible bird’s nest. Food
Research International 140: 109875.
Divean, S. & Tan, C.S. 2022. Optimization of brushing, bubble,
and microbubble techniques using Taguchi method for raw edible bird nest
cleaning purpose. Pertanika Journal of
Science & Technology 3(2): 1273-1288.
Fan, Q., Lian,
J., Liu, X., Zou, F., Wang, X. & Chen, M. 2022. A study on the skin
whitening activity of digesta from edible bird’s
nest: A mucin glycoprotein. Gels 8(1): 24.
Hwang, E., Park, S. & Yang
J-E. 2020. Anti-aging, anti-inflammatory, and wound-healing activities of
edible bird’s nest in human skin keratinocytes and fibroblasts. Pharmacognosy Magazine 16(69): 336-342.
Jaffar, F.H.F., Osman, K., Chua, K.H., Zulkefli,
A.F. & Ibrahim, S.F. 2021. Edible bird’s nest supplementation improves male
reproductive parameters of Sprague Dawley rat. Frontiers in Pharmacology 12: 631402.
Jamalluddin, N.H., Tukiran, N.A., Ahmad Fadzillah, N. & Fathi, S.
2019. Overview of edible bird’s nests and their contemporary issues. Food
Control 104: 247-255.
Kew, P.E., Wong, S.F., Lim, P.K.
& Mak, J.W. 2014. Structural analysis of raw and
commercial farm edible bird nests. Tropical Biomedicine 31(1): 63-76.
Kim, H.M., Lee, Y.M., Kim,
E.H., Eun, S.W., Sung, H.K., Ko,
H., Youn, S.J., Choi, Y., Yamada, W. & Shin, S.M.
2022. Anti-wrinkle efficacy of edible bird’s nest extract: A randomized,
double-blind, placebo-controlled, comparative study. Frontiers in
Pharmacology 13: 843469.
Lai, Q.W.S., Guo, M.S.S., Wu, K.Q., Liao, Z., Guan, D., Dong, T.T.,
Tong, P. & Tsim, K.W.K. 2021. Edible bird’s nest,
an asian health food supplement, possesses
moisturizing effect by regulating expression of filaggrin in skin keratinocyte. Frontiers in Pharmacology 12: 685982.
Lian, J-M., Fan, Q-Y. & Li, H-W. 2017. Influence of
different processing technology on sialic acid content of edible bird’s nest
products. 食品工业科技1: 265-268.
Lim, C.T.S., Norhafizah, M., Sani, D. Tan, S.N., Lim, C.W., Kirby, B.P., Ideris, A. & Stanslas,
J. 2021. Edible bird nest protects the kidney from gentamicin induced acute
tubular necrosis. Frontiers in Pharmacology 12: 726005
Loh, S-P., Cheng, S-H. & Mohamed, W. 2022. Edible bird’s
nest as a potential cognitive enhancer. Frontiers in Neurology 13:
865671.
Goh K.M., G.K., Lai, W.K.,
Ting, P.H., Koe, D. & Wong, J.K.R. 2017. Size
characterisation of edible bird nest impurities: A preliminary study. Procedia
Computer Science, Knowledge-Based and Intelligent Information &
Engineering Systems: Proceedings of the 21st International Conference,
KES-20176-8 September 2017, Marseille, France. 112: 1072-1081.
Murugan, D.D., Zuhaida, M.Z., Choy, K.W.,
Nor Hisam, Z., Choong,
M.J., Lim, Y.M. & Mohd Rais,
M. 2020. Edible bird’s nest protects against hyperglycemia-induced
oxidative stress and endothelial dysfunction. Frontiers in Pharmacology 10: 01624.
Ningrum, S., Candra, A. & Wardhani, H.C. 2023. The potency of Citrus aurantiifolia swingle and sea
salt solution as a cleansing agent for edible bird’s nests. Makara Journal
of Science 27(1): Article 4.
Quek, M.C., Chin, N.L., Yus Aniza, Y., Law, C.L. & Tan, S.W. 2018. Characterization
of edible bird’s nest of different production, species and geographical origins
using nutritional composition, physicochemical properties and antioxidant
activities. Food Research International 109: 35-43.
Ramachandran, R., Babji, A. S. & Wong, I.P. 2017. Effect of Heating on Antioxidant Activity on Edible Bird Nest. The 7th international seminar on tropical animal production (ISTAP): 380-386.
Shim, E.K-S. & Lee, S-Y.
2020. Calcite deposits differentiate cave from house-farmed edible bird’s nest
as shown by SEM-EDX, ATR-FTIR and Raman microspectroscopy. Chemistry - An Asian Journal 15(16): 2487-2492.
Subramaniam, Y., Yeong, C.F. & Ming,
E.S.L. 2015. Edible bird nest processing using machine vision and robotic arm. Jurnal Teknologi 72(2): 85-88.
Susilo, H., Latif, H. & Ridwan, Y.
2016. Application of washing method under running water to reduce nitrit level of edible bird’s nest. Jurnal Kedokteran Hewan -
Indonesian Journal of Veterinary Sciences 10(2): 95-97.
Tai, S.K., Hamzah,
Z., Ng, Q.H. & Tan, C.S. 2020. Surface morphology study on unclean,
commercial and bromelain treated edible bird nest (EBN) using scanning electron
microscope. IOP Conference Series: Materials Science and Engineering 932(1): 012013.
Tan, H.Y., Lim, S.J., Abdul
Salam, B., Muhamad Hanif, R. & Shahrul Razid, S. 2021. Enzymatic
hydrolysis: Sialylated mucin (SiaMuc)
glycoprotein of edible swiftlet’s nest (ESN) and its molecular weight
distribution as bioactive ESN SiaMuc-glycopeptide hydrolysate. International Journal of Biological Macromolecules 175:
422-431.
Tan, H.Y., Mun,
S.L., Lee, J.L., Lim, S.J., Nur Aliah,
D., Abdul Salam, B., Muhamad Hanif, R. & Shahrul Razid, S.L. 2022.
Bioactive sialylated-mucin (SiaMuc) glycopeptide produced from enzymatic hydrolysis of
edible swiftlet’s nest (ESN): Degree of hydrolysis, nutritional
bioavailability, and physicochemical characteristics. International Journal
of Food Properties 25(1): 252-277.
Tan, K.H. 2022. Applying best
value approach in establishing guideline for edible bird nest cleaning process.
PhD Dissertation, UTAR (Unpublished).
Utomo, B., Rosyidi, D., Radiate, L.E.
& Purnomo, H. 2016. Metode penurunan kandungan nitrite dengan pencucian menggunakan asam askorbat pada tiga jenis sarang burung walet asal Indonesia. Efektor 3(1): 104-107.
Utomo, B., Rosyidi, D., Radiati, L.E., Tri Puspaningsih,
N.N. & Proborini, W.D. 2018. Use of keratinase to
maintain pre-washing glycoprotein profiles of edible bird’s nest. Drug
Invention Today 10: 2986-2990.
Yeo, B.H., Rukayadi,
Y., Lai, O-M., Wong, S-F. & Tan, C-P. 2023. Structural and chemical
analysis of different types of raw cleaned (RC) edible bird’s nest (EBN). Malaysian
Journal of Microscopy 19(1): 76-89.
Yeo, B-H., Tang, T-K., Wong,
S-F., Tan, C-P., Wang, Y., Cheong, L-Z. & Lai, O-M. 2021. Potential
residual contaminants in edible bird’s nest. Frontiers in Pharmacology 12: 631136.
*Pengarang untuk surat-menyurat; email: omlai@upm.edu.my
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